Fine-Mapping of qECL7.1, a Quantitative Trait Locus Contributing to Epicotyl Length in Adzuki Bean (Vigna angularis)

Increasing the epicotyl length (ECL) of adzuki bean cultivars enhances suitability for mechanical weeding during vegetative stages and harvesting at pod maturity. To explore genetic control ECL, to identify molecular markers that could facilitate breeding increased recombinant inbred lines (RILs) were developed from a cross between Toiku161 (long epicotyls) Chihayahime (ordinary epicotyls). In this study, four quantitative trait loci (QTLs) identified ECL by QTL-seq analysis, one each on chromosomes 2, 7, 10 11. Insertion deletion (InDel)-based mapping also detected QTLs qECL7.1, 10, qECL10.1. Substitution using InDel, cleaved amplified polymorphic sequence (CAPS), derived (dCAPS), single nucleotide polymorphism (SNP) narrowed chromosomal location qECL7.1 418 kb region flanked DNA TC99_10,211,134 bp TC102_10,628,880 bp. A total 35 genes predicted within region. The here will contribute towards marker-assisted selection desirable long genotypes allow mechanization more efficient production.